2015 UMB/CMMS Current EM Techniques Workshop
** REGISTRATION CLOSED **
The aim of the Current EM Techniques Workshop is to introduce new electron microscopy techniques and instrumentations via live demonstration and open discussion.
Date: May 28 -29, 2015
Location: University of Maryland, Baltimore, Health Science Facility II Auditorium and Electron Microscopy Core Imaging Facility (EMCIF)
Format: Scientific presentations, live demonstrations, personal sample tryout, tips and tricks discussion forum led by technical experts.
Social with Local Microscopy Society: Chesapeake Microscopy and Microanalysis Society (CMMS) dinner meeting on May 28, 2015, 5:30 to 8:30 PM; Gladhill Boardroom, UMB HS/SL 5th floor.
Keynote speaker: Dr. Kent McDonald, UC Berkeley, "Mythbuster: The Electron Microscopy Episode"
Application talk1: Dr. Kirk Czymmek, Carl Zeiss Microscopy, LLC. "Workflows for Fluorescent Affinity Probes with Correlative Array Tomography"
Application talk2: Dr. Peter Van de Plas, Aurion. "Immunogold/Silver staining: post and pre-embedding applications"
Application talk3: Dr. Rick Powell, Nanoprobes. "Nanogold, Other EM labels, and Alternatives to antibody labeling"
- Station 1: High pressure freezer, EMPACT 2/Automated Freeze substitution system
- Station 2: Cryo-ultramicrotome
- Station 3: CryoTEM and Grid plunger
- Station 4: CryoSEM and Cryotransfer holder
- Station 5: CorrSight CLEM Solution presentation
- Station 6: Quick freeze substitution system
Station 1:Drs. Levi Felts & Mark Kukucka, Leica Microsystems
Station 2:Ms. Carol Cooke, Johns Hopkins University; Ms Johanna Sotiris, UMB
Station 3:Dr. Ulrich Baxa, NIH/NCI; Siddarth Raman, UMB & Daniel Phillips UMB
Station 4: Mr. Rod Heu, Colgate-Palmolive Technology Center; John Strong, UMB
Station 5: Dr. Gregor Heiss & Tim Maitland, FEI. Co
Station 6: Dr. Kent McDonald, UC Berkeley; Shilinag (Stevens) Zhang, NIH/NIDA
Tips and Tricks Discussion Forums:
|9:00 - 9:15||Ru-ching Hsia||Tokuyasu immunogold labeling using monoclonal antibodies|
|9:15 - 9:30||Shiliang (Stevens) Zhang||Pre-embedding immunolabeling of rodent brain|
|9:30 - 9:45||Ron Petralia||Pre-embedding immunoperoxidase vs. gold techniques|
|9:45 -10:00||Rick Powell||Gold and silver enhancement and troubleshoot|
|10:00 -10:30||Coffee/Tea break|
|10:30 -10:45||Ron Petralia||Post-embedding immunogold labeling using freeze substitution of fixed tissue|
|10:45 -11:00||Kunio Nagashima||In situ post-embedding IEM for correlated microscopy|
|11:00 -11:15||Peter Van de Plas||Immunogold silver staining: troubleshooting|
|11:15- 12:00||Rick Powell||Nanogold, Other EM Labels, and Alternatives to Antibody Labeling|
Dr. Peter Van de Plas, Aurion
Mr. Kunio Nagashima, Leidos EM Lab
Dr. Ru-ching Hsia, EMCIF, University of Maryland, Baltimore
Dr. Ronald Petralia, Advanced Imaging Core, NIH/NIDCD
Dr. Rick Powell, Nanoprobes
Dr. Shiliang (Steven) Zhang, Electron Microscopy Core, NIH/NIDA
CMMS Spring dinner speaker:
Dr. Kent McDonald, UC Berkeley, " A History of cryomethods in Biological Electron Microscopy"
Dr. John Cumings, Department of Materials Science and Engineering, University of Maryland College Park, " "In Situ Electron Microscopy of Battery Nanomaterials"
CMMS Spring Dinner Agenda and Information
$275 Full workshop including CMMS dinner meeting
$250 Workshop only
$30 CMMS dinner only ($35, pay at the door)
Pleas check out our other workshops and courses in 2015
Travel: Workshop participants are strongly encouraged to use various modes of public transportation to reach UMB. If you wish to drive to the workshop, Penn Street Garage or Pratt Street Garage are the closest parking garages. Parking vouchers will be available to registered workshop participants on the day of the workshop. Please take a ticket when you drive into the garage and pay with the voucher when you exit
Lodging: if you need assistance for hotel reservations, please email: UMBCurrentEMWorkshop@umaryland.edu
Sponsors: Angstrom, Aurion, Carl Zeiss Microscopy, Chesapeake Microscopy and Microanalysis Society (CMMS), EMS-Diatome, FEI.Co, Jeol, Leica Microsystems, Nanoprobes, Ted Pella, Inc., Tescan USA Inc., Tousimis.