Cryo EM Sample Prep Minicourse

muscleSeptember 17 to 18, 2019


Location: University of Maryland Baltimore, Electron Microscopy Core Imaging Facility (EMCIF)

Instructors and demonstrators: 

Ru-ching Hsia, EMCIF, UMB
Shigeki  Watanabe, Johns Hopkins University
Nancy Rizzo, Leica Microsystems
Mark  Kukucka, Leica Microsystems

Scope of aims:  This is an introductory course to introduce the knowledge and skills of various cryo TEM/SEM sample preparation techniques, including high pressure freezing, plunge freezing and cryo-ultramicrotomy

Targeted participants:  Individuals who wish to learn cryo EM sample preparation of biological specimen.  No experience is required. 

Format: Lecture, demonstration, and hands-on practice

Techniques covered: Cryo-immobilization, High Pressure freezing, Automated freeze substitution, Cryo-ultramicrotomy, Grid plunging, Rapid freeze substitution, etc.

Instrumentation:  High pressure freezer ICE and EMPACT II (Leica-Microsystems), AFS (Leica-Microsystems), Grind plunger  (Leica-Microsystems), Cryo-ultramicrotome UC6/FC7 (Leica-Microsystems)

Agenda_2019CryoEMSamplePrep (tentative)

Registration Fee: $600 

10% Discount for UMB researchers, members of Chesapeake Microscopy Microanalysis Society (CMMS) and returning participants or multiple course registration.

Please indicate discount eligibility on registration form.

Participants: maximum 6


Registration will be closed when all spaces are filled.

An invoice with payment instruction will be e-mailed to you within five -ten business day upon receipt of registration.

A list of nearby hotels that offer a special rate for UMB guests is available upon request, please contact EMCIF.

Payment Method: Registration fee can be paid by check or credit card.  Follow the instruction on the invoice.

 Inquiry: Email