Cryo EM Sample Prep Minicourse
September 17 to 18, 2019
Location: University of Maryland Baltimore, Electron Microscopy Core Imaging Facility (EMCIF)
Instructors and demonstrators:
Ru-ching Hsia, EMCIF, UMB
Shigeki Watanabe, Johns Hopkins University
Nancy Rizzo, Leica Microsystems
Mark Kukucka, Leica Microsystems
Scope of aims: This is an introductory course to introduce the knowledge and skills of various cryo TEM/SEM sample preparation techniques, including high pressure freezing, plunge freezing and cryo-ultramicrotomy
Targeted participants: Individuals who wish to learn cryo EM sample preparation of biological specimen. No experience is required.
Format: Lecture, demonstration, and hands-on practice
Techniques covered: Cryo-immobilization, High Pressure freezing, Automated freeze substitution, Cryo-ultramicrotomy, Grid plunging, Rapid freeze substitution, etc.
Instrumentation: High pressure freezer ICE and EMPACT II (Leica-Microsystems), AFS (Leica-Microsystems), Grind plunger (Leica-Microsystems), Cryo-ultramicrotome UC6/FC7 (Leica-Microsystems)
Agenda_2019CryoEMSamplePrep (tentative)
Registration Fee: $600
10% Discount for UMB researchers, members of Chesapeake Microscopy Microanalysis Society (CMMS) and returning participants or multiple course registration.
Please indicate discount eligibility on registration form.
Participants: maximum 6
REGISTER Now
Registration will be closed when all spaces are filled.
An invoice with payment instruction will be e-mailed to you within five -ten business day upon receipt of registration.
A list of nearby hotels that offer a special rate for UMB guests is available upon request, please contact EMCIF.
Payment Method: Registration fee can be paid by check or credit card. Follow the instruction on the invoice.
Inquiry: Email coreimaging@umaryland.edu