Sponsored by Leica-Microsystems
Baltimore, Maryland Click on the links below to watch the lectures and equipment demonstration video - Preservation methods and the electron tomography revolution in cellular 3D structuresby Dr. Gina Sosinsky,
Cryo preparation Pathways to Co-relative Microscopyby Dr. Kim Rensing, Leica-Microsystems
- High pressure freezer Leica EMPACT 2 and freeze substitution Leica AFS 2 Demonstration
The purpose of this workshop is to discuss the rapidly evolving new techniques for high pressure freezing, freeze substitution and cryosectioning. The use of correlative LM and EM techniques will be shown via a "Live" video format. There will be a special emphasis on step by step procedures. Topics of discussion will include: - How to optimize freezing for a wide variety of samples
- Strategies for freeze substitution
- How to cut cryosections of frozen hydrated and sucrose infiltrated samples
- Live cell imaging
- Tomography
Individuals participating in this session should leave with a working knowledge of these cryo-techniques that they can apply immediately to their own research, whether it involves tomography, cryo-sectioning, EM immuno-labeling, or if they just want to have the best available preservation of cellular fine structure. University of Maryland, Baltimore is centrally located in downtown Baltimore and can be easily accesse by public transportation Limited parking coupons will be available for registered workshop participants, please indicate in your registration form. Parking information.... Printable Format  March 16, 2009 | 9:00-9:30
Ground floor
atrium | Registration & sample collection | 9:30-10:30
G205 | Dr. Gina Sonsinsky, UCSD
Preservation methods and the electron tomography revolution in cellular 3D structures | 10:30-11:00 | Break | 11:00-12:00
G205 | Dr. Kim Rensing, Leica-Microsystem
Cryo preparation Pathways to Co-relative Microscopy | 12:00-1:00 | Lunch, pizzza will be provided |
1:00-1:30
1:30-2:00
2:00-2:30
Break
3:00-3:30
3:30-4:00
4:00-4:30
| Instrument Demonstrations
Station 1 High Pressure Freezer (EMPACT 2). Room 8404
Station 2 Freeze substitution(Leica EM AFS2). Room 8404
Station 3 Cryo-ultramicrotome(Leica EM UC6) Room 8404
Station 4 Inverted microscope Room 9102
Station 5 Ultramicrotome & Immunogold labeling system Room 9405
Station 6 DVD demonstration & live cast Room 9102, Room 9105, Room 9106 Each demonstration is 30 min, total 6 sessions per demonstration
Check your group color for schedule | | 4:30-5:00 | Rountable discussion for hands-on | Joint Dinner event with Chesapeake Microscopy Society (CMS)
Catered dinner @ Dental school ground floor atrium
Separate registration required | | 6:00-6:30 | Social & Drinks | | 6:30-7:30 | Joint dinner event with Chesapeake Microscopy Society | | 7:30-8:30 | D. Thomas Blanpied, University of Maryland
Imaging the dynamic organization of living synapses | March 17, 2009 | 9:00-9:30 | Roundtable clinic: Immunolabeling | 9:30-10:00 | Roundtable clinic: ultramicrotomy, EM sample processing | 10:00-10:30 | Break | | 10:30-12:00 | Hands-on, Room 8404 | | 12:00-1:00 | Pizza, Room 9102 | 1:00-5:00 | Hands-on, Room 8404 |
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