updated 02/11/09
The Electron Microscopy Core Facility strives to provide individual services that fit each user’s specific needs. The routine services we provide include: | --Preparation of fixatives | | --Preparation of tissues for scanning electron microscopy | | -Cirtical point drying | | -HMDS drying | | -Mounting | | -Sputter coating | | --Processing and embedding of tissues, cultured cells or microorganisms in epoxy resins or acrylics | | --Thick one micron sectioning w toludine blue stain | | --Ultrathin sectioning plastic embedded material or frozen sample | | --Immunolabelling | | -Post embedding method | | -Pre-embedding method | | --Negative staining | | --Formva or Fromva/carbon coated grids | | --Imaging using the transmission and scanning electron microscopes | | --Image analysis |
More advanced EM techniques such as cryo-sectioning, wet and cryo SEM and freeze fracture are also avaialbe. Contact us if you have any question about electron microscopy related research.
The best way to save the cost of EM services is to learn the technique and do it yourself. We conduct regular trainings to help our facility members learn to perform EM related experiments. Basic scope operation training: This training is required to all users (members or non-members) who wish to operate scanning electron microscope or transmission electron microscope. SEM and TEM regular basic scope training is conducted by appointment only. EM techniques training: This training is only available for facility members. Techniques include: negative staining, immunogold labeling, EM sample prepartion, etc. Advanced EM operation training. This training is only avialalbe to facility members who have taken basic training and have more than 20 hours of independent scope usage experience. Users have to be certified by the facility staff before advanced training can be scheduled. Users who are certified as advanced scope users can access the EM faciliy off regular open hours. Contact us to schedule your training session.
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