Confocal Microscope, inverted
Radiance 2100, BioRad
|Core Imaging Facility|
The BioRad Radiance 2100 is a laser scanning confocal microscope capable of multichannel fluorescnece, reflectance and transmitted light imaging. The scan head is attached to a Nikon Eclipse TE300 inverted microscope, which has optics and filters for fluorescence and DIC applications.
Features and Specifications
Nikon Plan 10X / 0.25, Ph1, DL
Nikon PlanFluor 20X / 0.50, Ph1, DLL, WD 2.1
Nikon PlanFluor 40X / 0.75, Ph2, DLL, WD 0.72
Nikon PlanApo 60X A/ 1.40 Oil, DIC H, WD 0.21
Nikon PlanApo 100X/ 1.40 Oil, DIC H , WD 0.13
Argon Laser (457, 477, 488, 514 nm)
HeNe laser (543 nm)
Red laser diode (637 nm).
|Laser line||Fluorophore type||Examples|
|457 nm||Cyan||Alexa Fluor 430, BO-PRO-1, CFP|
|476 nm||Green||DiO, FM 1-43, NBD-X|
|488 nm||Green||Alexa Fluor 488, Calcein, Cy2, eGFP, FITC, Oregon Green, YO-PRO-1|
|514 nm||Yellow||Alexa Fluor 514, EthD-1, YFP|
|543 nm||Red||Alexa Fluor 546, 555, and 568, Cy3, DiI, DsRed, Phycoerythrin (PE), Propidium Iodine (PI), Rhodamine, Texas Red, TRITC|
|637 nm||Far Red||Alexa Fluor 633 and 647, Allophycocyanin (APC), Cy5, TO-PRO-3|
The LaserSharp operating software allows simultaneous and sequential collection of 2D, 3D, and 4D images that can be collected in a horizontal plane, vertical plane, xyz horizontal stack, or a timed sequence. Stacks and timed sequences can be used to create an animation series.
The Radiance 2100 is charged at $20 per hour at an hourly increment. For more information or scheduling scope training, please contact CIF Director.
Scope Availability and Reseravation Fee
Registration and scope training are required before users are allowed to use the CIF scopes and instrument. Registered users will be assigned user name and password to access CIF server and scope reservation calendar. Follow the link below for registration and scope reservation. Please contact CIF Director to schedule scope trainings.