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Grid Plunger, EM GP

Leica Microsystem
Core Imaging Facility

alto 2100 cryo transfer chamber

The Leica EM GP can be used for the vitrification of thin samples (up to a few microns) like viruses, proteins and cellular components as well as adherent cells for cryo-TEM and cryo electron tomography.  Samples in suspension are applied onto grids in a humidity controlled environmental chamber and  plunge freezen into liquid ethane after removing excess fluid by automatic blotting. This is the bare grid technique.


  • Prior to freezing, the sample is maintained in a temperature and humidity controlled environmental chamber, adjustable between
    +4° and +60°C and room humidity to 99 %
  • Grids are  blotted single sided to prevent damge to films. 
  • Blotting can be done  automatically or manually.
  • Manual (motorised) or automatic (programmed) 180° grid rotation allows blotting from either side of the grid.

This instrument is purchased with funding from a NIH S10 Shared Instrument award.

GP1


 
 
cryoSEM, kidney



 
GP4


 
 

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