Date: October 17-18, 2016
Location: University of Maryland Baltimore, Electron Microscopy Core Imaging Facility (EMCIF)
Instructor: Dr. Helmut Gnaegi, Diatome Ltd
Lecturer and demonstrator: EMCIF Director (Dr. Ru-ching Hsia) and staff
Scope of aims: To promote the knowledge and skills of advanced cryo-ultramicrotomy techniques
Targeted participants: Individuals who are experienced with ultramicrotome operation at ambient temperature and wish to extend their skills to include sectioning CEMOVIS and Tokuyasu immunolabeling specimen
Format: Lecture, demonstration and hands-on practice
Techniques covered: High pressure freezing of samples for Cryo electron microscopy of vitreous sections (CEMOVIS), Cryo-sectioning of CEMOVIS samples, Cryo sectioning of sucrose infused biological sample (for Tokuyasu immunolabeling)
Instrumentation: High pressure freezer ( Leica EMPACT II); Cryoultramicrotome (Leica UC6/FC7); Two Micromanipulator (EMS/Diatome)
Companion IGSS workshop on October 19 to 21 will cover Tokuyasu immunolabeling techniques along with post-embedding and pre-embedding immunogold labeling techniques. Participants of cryo ultramicrotomy course that are interested to learn the whole Tokuyasu immunolabeling methods are strongly encourage to attend IGSS workshop as well.
Course agenda will be available soon
Registration Fee: $600 ($550 if also attend Aurion IGSS workshop on 10/19-10/21, 2016)
Participants: maximum 6
Regisstration will close when all spaces are filled.
An invoice will be e-mailed to you within five business days upon the receipt of registration.
Registration can be paid by check or credit card.
Lodging: A list of nearby hotels that offer special rate for UMB guests is available upon request, please contact EMCIF.
Other inquiry: E-mail firstname.lastname@example.org
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